Endosymbiotic adaptations in three new bacterial species associated with Dictyostelium discoideum: Paraburkholderia agricolaris sp. nov., Paraburkholderia hayleyella sp. nov., and Paraburkholderia bonniea sp. nov
First Author Brock
Last Author Strassmann
Journal PeerJ
Year 2020
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QSID Alternate ID Species Origin Location Origin Coordinates Box Plated From Date Collected Date Frozen Collection Method Culture Method Origin Soil Sample Location in Freezer Box Name Box Initials Clonal Mating Type violHapGrp Sequenced Amoebophilis Endosymbiont Neochlamydia Endosymbiont Neochlamydia Haplotype Endosymbiont Haplotype ID Endosymbiont 16S Sequence Hit Paraburkholderia Species
QS317 HD49A1 Dictyostelium discoideum Texas- Houston Arboretum N 29° 46’, W 95° 27’ Rack 18 - Box 8   July 15, 2004 29-3 VA 2000 AF Box 3 of 4 AF 3 chlam1 Pa. agricolaris
QS433 V303A1 Dictyostelium discoideum Virginia- Mountain Lake Biological Station N 37° 21’, W 80° 31’ Rack 29 - Box 8 Oct. 15, 2000 July 21, 2004 Soil V303 18-6 Natasha, Tiffany, Clea Msat Evol Proj. Clones from Meg's thesis July 21 2004 Box 1 CS 3 Pa. bonniea
QS859 7P 7.1 Dictyostelium discoideum Virginia- Mountain Lake Biological Station N 37.37613 W 080.5192 5E - 1 July 23, 2014 Aug. 13, 2014 deer feces slurry Eisenberg1976modified Set1 Site7 Wilson302-80C 5E - 1   B.bryophila Burkholderia bryophila/terricola 99% Pa. bonniea
QS983 5S 7.1 Dictyostelium discoideum Virginia- Mountain Lake Biological Station N 37.37612 W 080.51909 5E - 4 July 23, 2014 Aug. 13, 2014 trowel Eisenberg1976modified Set1 Site5 Wilson302-80C 5E - 4   chlam1 B.fungorum 2 Burkholderia fungorum haplotype 2 Pa. agricolaris
QS1007 9S 7.1 Dictyostelium discoideum Virginia- Mountain Lake Biological Station N 37.37617 W 080.51926 5E - 5 July 23, 2014 Aug. 13, 2014 trowel Eisenberg1976modified Set1 Site9 Wilson302-80C 5E - 5   messy